The Bio-X community was invited to join Chip Beckwith from Agilent Technologies and learn different qPCR assay types and how to select which is best for your application, what to consider when designing a qPCR assay, suggestions for optimizing data quality (i.e. when to use a reference dye, normalizing gene, etc), about MIQE guidelines for q-RT-PCR data by ensuring RNA quality, and about the use of two tools for NGS library quant.

Event agenda:

1:00pm

Introduction to qPCR, Assay Optimization and Normalization

2:00pm

Coffee break

Importance of RNA quality in downstream QPCR applications

Validation of Microarray Data by qPCR

Next Generation Library Quantization via qPCR