2017 Undergraduate Summer Research Program Participant

Home Department: Biology
Supported by: Bio-X
Mentor: Laura Attardi, Radiation Oncology and Genetics

Photo of USRP student Clare Moffatt in the lab, holding up a tray of cultures.The transcription factor p53 is critical in suppressing tumorigenesis in humans and mice. The Attardi lab has used affinity purification and mass spectrometry to identify novel transcriptional co-repressors of mouse p53, and one of these is the protein Spen, which is known as a negative regulator of cancer signaling pathways. Clare will be validating the interactions between p53 and Spen using co-immunoprecipitation assays and then determining how Spen may function as a co-repressor of p53 target gene expression and in tumor suppression through Spen inhibition.

Poster presented at the Stanford Bio-X Interdisciplinary Initiatives Symposium on August 24, 2017:

Characterization of the p53 Tumor Suppressor Protein-Protein Interactions Identified by Affinity Purification and Mass Spectrometry

Clare Moffatt1, Nitin Raj1, Nancie Moonie2, Janos Demeter2, Ahlima Roumane1, Sara Sakowitz1, Peter Jackson2, Laura Attardi1
[Departments of Radiation Oncology1 and Microbiology & Immunology2, Stanford University]