Home Department: Biology
Supported by: Bio-X
Mentor: Laura Attardi, Radiation Oncology and Genetics

The transcription factor p53 is critical in suppressing tumorigenesis in humans and mice. The Attardi lab has used affinity purification and mass spectrometry to identify novel transcriptional co-repressors of mouse p53, and one of these is the protein Spen, which is known as a negative regulator of cancer signaling pathways. Clare will be validating the interactions between p53 and Spen using co-immunoprecipitation assays and then determining how Spen may function as a co-repressor of p53 target gene expression and in tumor suppression through Spen inhibition.

Poster presented at the Stanford Bio-X Interdisciplinary Initiatives Symposium on August 24, 2017:

Characterization of the p53 Tumor Suppressor Protein-Protein Interactions Identified by Affinity Purification and Mass Spectrometry

Clare Moffatt¹, Nitin Raj¹, Nancie Moonie², Janos Demeter², Ahlima Roumane¹, Sara Sakowitz¹, Peter Jackson², Laura Attardi¹
[Departments of Radiation Oncology¹ and Microbiology & Immunology², Stanford University]