Awarded in 2017
Home Department: Chemistry
Faculty Advisors: Alice Ting (Genetics, Biology) and Howard Chang (Dermatology)
Research Title: Proximity biotinylation for spatially resolved RNA sequencing in living cells
Research Description: Spatial regulation of RNAs plays a fundamental role in cell development, brain function, and a wide variety of diseases including Parkinson’s. However, current technologies for mapping RNA localization have limitations; for example FISH/merFISH have limited throughput and require sophisticated instrumentation. Using both chemical and cell biological approaches, Shuo proposes to develop an alternative method based on the engineered peroxidase enzyme APEX. Shuo envisions that this alternative method, followed by enrichment and RNA sequencing, will generate nanometer-resolution spatial maps of functionally important RNAs.