Home Department: Bioengineering
Mentor: Thomas Südhof, Molecular & Cellular Physiology

“A High-Throughput Deep Learning Pipeline for CRISPR gRNA Design to Screen a Library of Neuronal Adhesion Molecules”

Understanding the logic that underlies synapse formation and specificity is integral to unraveling the molecular bases of our thoughts, memories, and behaviors. Synapse formation and regulation are orchestrated with high spatiotemporal precision in processes mediated by adhesion and signaling between transmembrane proteins. In his 2020 Stanford Bio-X research project, Sid will analyze a library of expressed neuronal transmembrane proteins and design repair templates to tag each protein using a novel CRISPR-based HDR strategy. In the future, these designs may be used to identify molecules of interest and track their localization at the synapse.