2017 Undergraduate Summer Research Program Participant

Home Department: Chemistry
Supported by: Anonymous Donor
Mentor: Ronald Davis, Biochemistry and Genetics

With the development of CRISPR-Cas9 mediated gene editing technology, the world of scientific and medical research has been given an incredibly powerful tool. Tucker will be optimizing a new high throughput editing system for improved efficiency and mismatch tolerance using high fidelity CRISPR systems. Higher fidelity genetic engineering will ensure the safety and reproducibility of these editing systems as they are employed at ever larger scales. An understanding of the fidelity of these CRISPR systems will help ensure that only the intended genomic changes are made.

Poster presented at the Stanford Bio-X Interdisciplinary Initiatives Symposium on August 24, 2017:

Dissecting Mechanisms of Drug Action by Structure-Function Mapping with CRISPR Saturation Editing of Amino Acids

Tucker Burnett1, Kevin Roy2, Justin Smith2,3, Maddison Morgan4, Julia Schulz5, Kevin Orsley6, Lars Steinmetz2, Bob St. Onge3, Ron Davis2,3
[Departments of Chemistry1, Genetics2, Biochemistry3, and Bioengineering5, Stanford University; Department of Biology4, Middlebury College; Department of Biology6, Emmanuel College]