2017 Undergraduate Summer Research Program Participant

Home Department: Bioengineering
Supported by: Bio-X
Mentor: Lars Steinmetz, Genetics

Protein phosphorylation is essential for cellular process, and deregulation in phosphorylation networks is linked to the progression of cancer. Julia’s project will utilize massively parallel genome editing, which uses CRISPR Cas9 to systematically mutate all phosphosites in the yeast proteome, and assess the phenotypic consequences in a variety of stress conditions. This will help us to understand the functional architecture of cell signaling networks and give insight into how deregulation results in cancer progression.

Poster presented at the Stanford Bio-X Interdisciplinary Initiatives Symposium on August 24, 2017:

Improving the Efficiency of Homologous Recombination in High-Throughput CRISPR Editing inSaccharomyces cerevisiae

Julia Schulz1, Justin D. Smith2,3, Kevin Roy2, Maddison Morgan4, Tucker Burnett5, Kevin Orsley6, Sundari Suresh3, Angela Chu3, Ron Davis2,3, Bob St. Onge3, Lars Steinmetz2
[Departments of Bioengineering1, Genetics2, Biochemistry3, and Chemistry5, Stanford University; Department of Biology4, Middlebury College; Department of Biology6, Emmanuel College]