Home Department: Bioengineering
Supported by: Bio-X
Mentor: Lars Steinmetz, Genetics

Protein phosphorylation is essential for cellular process, and deregulation in phosphorylation networks is linked to the progression of cancer. Julia’s project will utilize massively parallel genome editing, which uses CRISPR Cas9 to systematically mutate all phosphosites in the yeast proteome, and assess the phenotypic consequences in a variety of stress conditions. This will help us to understand the functional architecture of cell signaling networks and give insight into how deregulation results in cancer progression.

Poster presented at the Stanford Bio-X Interdisciplinary Initiatives Symposium on August 24, 2017:

Improving the Efficiency of Homologous Recombination in High-Throughput CRISPR Editing inSaccharomyces cerevisiae

Julia Schulz¹, Justin D. Smith^2,3, Kevin Roy², Maddison Morgan⁴, Tucker Burnett⁵, Kevin Orsley⁶, Sundari Suresh³, Angela Chu³, Ron Davis^2,3, Bob St. Onge³, Lars Steinmetz²
[Departments of Bioengineering¹, Genetics², Biochemistry³, and Chemistry⁵, Stanford University; Department of Biology⁴, Middlebury College; Department of Biology⁶, Emmanuel College]