Stanford Bio-X Seminar

Lucien Weiss, Stanford Bio-X Bowes Fellow and Zuckerman Postdoctoral Fellow, Technion, Israel Institute of Technology

Imaging fluorescently-labeled DNA in live cells with nanoscale precision shows significant promise as a diagnostic tool; however, the intrinsically stochastic nature of biological systems limits our ability to interpret meaningful signals from the noise. Here we discuss the implementation of advanced, 3D microscopy into an imaging flow cytometer and the unique calibration protocol we developed, in which we rely on statistical distributions rather than the unattainable static ground-truth. We demonstrate our system on live yeast cells, attaining 3D spatial information with orders of magnitude higher throughput than previous methods.

Featuring talks from Stanford Faculty:

 Tony Wyss-Coray
 Jamshid Ghajar
 Thomas Südhof

Featuring talks from Stanford Faculty:

 Euan Ashley
 Ash Alizadeh
 Melanie Hayden Gephart

Featuring talks from Stanford Faculty:

 Rajat Rohatgi
 Seung Kim
 Scott Dixon

Frontiers in Quantitative Biology Seminar

JENNIFER ZALLEN, MEMORIAL SLOAN KETTERING CANCER CENTER

The Zallen lab is using molecular, genetic, and cell biological approaches to understand the machinery that directs morphogenetic events. An understanding of the cell rearrangements that occur during normal embryonic development will uncover general principles that build tissues and organs and can provide insight into how deranged versions of these processes contribute to human disease.

Frontiers in Quantitative Biology Seminar

PAUL NURSE, THE FRANCIS CRICK INSTITUTE

The goals of Dr. Nurse's laboratory are to better understand the global cellular networks which regulate the eukaryotic cell cycle, cell form and cell growth. These cellular controls are fundamental to the growth, development and reproduction of all living organisms.

Frontiers in Quantitative Biology Seminar

ZEV GARTNER, UCSF

The Gartner lab seeks to answer questions about how tissue structure forms and functions. They take a synthetic approach, building human tissues from the bottom-up, which allows them to measure and perturb the molecular and physical properties of individual cells, reconstitute them into living tissue, then observe their interactions to reveal the underlying "rules" guiding their collective behaviors.

Frontiers in Quantitative Biology Seminar

JONATHAN WEISSMAN, UCSF

The Weissman laboratory is looking at how cells ensure that proteins fold into their correct shape, as well as the role of protein misfolding in disease and normal physiology. They are also developing experimental and analytical approaches for exploring the organizational principles of biological systems and globally monitoring protein translation through ribosome profiling.

Frontiers in Quantitative Biology Seminar

BRUCE EDGAR, UNIVERSITY OF UTAH

The Edgar lab uses genetics to characterize the programs of cell growth and proliferation that occur during development, regeneration and tumorigenesis, with the goal of finding the genes that act as limiting regulators in each context.

Frontiers in Quantitative Biology Seminar

BIL CLEMONS, CALTECH

The Clemons lab is primarily interested in understanding the molecular details of life and as a tool we focus on “structural biology”. They currently work on problems related to protein transport across membranes and post-translational modification of proteins. The lab primarily uses X-ray crystallography but also works with biochemistry, microbiology, mass spectrometry and electron microscopy.